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. 2018 Oct 31;38(44):9579–9599. doi: 10.1523/JNEUROSCI.0503-18.2018

Figure 2.

Figure 2.

Nestin and CaMK2α protein in brain (A–C, E) and placenta (D, F). Nestin expression in CON E13.5 brain A, B, Immunofluorescence localization of Nestin (A) showing corresponding Nomarski-differential interference contrast (DIC) images. Alexa Fluor 488-conjugated donkey anti-goat IgG (1/500) was applied after the goat anti-Nestin antibody (1/100) in an overnight incubation at 4°C. DAPI served as the nuclear stain. Nestin protein was expressed in E13.5 brain (B). Expression of CaMK2α in adult brain (C, E) and E18 placenta (D, F). Immunofluorescence localization of CaMK2α (E, F). Alexa Fluor 488-conjugated donkey anti-goat IgG (1/500 each, Jackson ImmunoResearch Laboratories) was applied after the goat anti-CaMK2α antibody (1/500) during an overnight incubation at 4°C. DAPI was the nuclear stain. C, D, Corresponding Nomarski differential interference contrast (DIC) images. E, F, CaMK2α protein was expressed in brain (E), not placenta (F).