Table 1.
SHIRPA primary screen score in CON (n = 34), HT (n = 19), and KO (n = 14) pups
| CON | HT | KO |
p-value |
|||
|---|---|---|---|---|---|---|
| (CON vs KO) | (CON vs HT) | (KO vs HT) | ||||
| Viewing jar | ||||||
| Body position | 2.70 ± 0.12 | 3 ± 0 | 2.43 ± 0.25 | 0.2962 | 0.5946 | 0.0377* |
| Spontaneous activity | 0.91 ± 0.05 | 0.95 ± 0.05 | 0.93 ± 0.07 | >0.9999 | >0.9999 | >0.9999 |
| Respiration rate | 2 ± 0 | 2 ± 0 | 1.93 ± 0.07 | 0.1993 | >0.9999 | 0.2914 |
| Tremor | 0 ± 0 | 0 ± 0 | 0 ± 0 | 1 | 1 | 1 |
| Urination | 0.059 ± 0.04 | 0.053 ± 0.05 | 0 ± 0 | >0.9999 | >0.9999 | >0.9999 |
| Defecation | 0.09 ± 0.06 | 0.16 ± 0.12 | 0.5 ± 0.2 | 0.0412 | >0.9999 | 0.1867 |
| Arena | ||||||
| Transfer arousal | 3.68 ± 0.19 | 4 ± 0.23 | 3.5 ± 0.34 | >0.9999 | 0.9389 | 0.6174 |
| Locomotor activity | 3.38 ± 0.57 | 3.89 ± 0.59 | 2.57 ± 0.72 | >0.9999 | >0.9999 | 0.6535 |
| Palpebral closure | 1.06 ± 0.04 | 1 ± 0 | 1.07 ± 0.13 | >0.9999 | >0.9999 | >0.9999 |
| Piloerection | 0 ± 0 | 0 ± 0 | 0 ± 0 | 1 | 1 | 1 |
| Startle response | 0 ± 0 | 0 ± 0 | 0 ± 0 | 1 | 1 | 1 |
| Gait | 0.68 ± 0.16 | 0.37 ± 0.21 | 1 ± 0.3 | 0.8883 | 0.8109 | 0.205 |
| Pelvic elevation | 1.94 ± 0.04 | 2 ± 0 | 2 ± 0.1 | >0.9999 | >0.9999 | >0.9999 |
| Tail elevation | 1 ± 0 | 1 ± 0 | 1 ± 0 | 1 | 1 | 1 |
| Touch escape | 1.06 ± 0.04 | 1 ± 0 | 1.07 ± 0.07 | >0.9999 | 0.9924 | >0.9999 |
| Positional passivity | 0 ± 0 | 0 ± 0 | 0 ± 0 | 1 | 1 | 1 |
| Trunk curl | 1 ± 0 | 1 ± 0 | 1 ± 0 | 1 | 1 | 1 |
| Limb grasping | 1 ± 0 | 1 ± 0 | 1 ± 0 | 1 | 1 | 1 |
| Whisker brush | 1.88 ± 0.06 | 2 ± 0 | 1.93 ± 0.07 | >0.9999 | 0.3737 | >0.9999 |
| Above arena | ||||||
| Abnormal behavior | 1 ± 0 | 1 ± 0 | 1 ± 0 | 1 | 1 | 1 |
| Visual placing | 1.91 ± 0.05 | 1.68 ± 0.11 | 1.64 ± 0.13 | 0.1094 | 0.1478 | >0.9999 |
| Grip strength | 1.70 ± 0.08 | 1.68 ± 0.11 | 1.21 ± 0.11 | 0.0039* | >0.9999 | 0.0152* |
| Wire manoeuvre | 2.38 ± 0.12 | 2.16 ± 0.09 | 3 ± 0.23 | 0.0148* | 0.7352 | 0.002* |
| Body tone | 1 ± 0 | 1 ± 0 | 1 ± 0 | 1 | 1 | 1 |
| Pinna reflex | 0.38 ± 0.08 | 0.47 ± 0.12 | 0.35 ± 0.13 | >0.9999 | >0.9999 | >0.9999 |
| Corneal reflex | 0.91 ± 0.05 | 0.95 ± 0.05 | 0.78 ± 0.11 | 0.6029 | >0.9999 | 0.4203 |
| Toe pinch | 1.15 ± 0.15 | 1.26 ± 0.15 | 1.21 ± 0.19 | >0.9999 | >0.9999 | >0.9999 |
| Supine restraint | ||||||
| Body length | 5.46 ± 0.07 | 5.25 ± 0.08 | 4.79 ± 0.12 | <0.0001* | 0.172 | 0.0049* |
| Skin color | 1 ± 0 | 1 ± 0 | 1 ± 0 | 1 | 1 | 1 |
| Limb tone | 1 ± 0 | 1 ± 0 | 1 ± 0 | 1 | 1 | 1 |
| Lacrimation | 0.06 ± 0.04 | 0 ± 0 | 0 ± 0 | 0.8524 | 0.7066 | >0.9999 |
| Salivation | 0 ± 0 | 0 ± 0 | 0 ± 0 | 1 | 1 | 1 |
| Provoked biting | 0.44 ± 0 | 0.31 ± 0.11 | 0.28 ± 0.13 | 0.9635 | >0.9999 | >0.9999 |
| Righting reflex | 0.20 ± 0.07 | 0.16 ± 0.09 | 0.5 ± 0.14 | 0.0995 | >0.9999 | 0.0773 |
| Contact righting | 1 ± 0 | 1 ± 0 | 1 ± 0 | 1 | 1 | 1 |
| Negative geotaxis | 2.53 ± 0.31 | 1.89 ± 0.45 | 2.86 ± 0.5 | >0.9999 | 0.7093 | 0.4363 |
| Fear | 0 ± 0 | 0 ± 0 | 0 ± 0 | 1 | 1 | 1 |
| Irritability | 0.97 ± 0.03 | 0.89 ± 0.07 | 1 ± 0 | >0.9999 | 0.6206 | 0.465 |
| Aggression | 0.41 ± 0.09 | 0.26 ± 0.1 | 0.28 ± 0.13 | >0.9999 | 0.8529 | >0.9999 |
| Vocalization | 0.03 ± 0.03 | 0 ± 0 | 0 ± 0 | >0.9999 | >0.9999 | >0.9999 |
| Body weight | 5.92 ± 0.12 | 5.31 ± 0.11 | 4.58 ± 0.2 | <0.0001* | 0.0064* | 0.0067* |
*Significant difference among the groups as analyzed by one-way ANOVA followed by Bonferroni's multiple-comparisons test.