. 2019 Aug 22;14(8):e0221040. doi: 10.1371/journal.pone.0221040

Table 5. Characteristics of the 10 polymorphic SSR markers and the novel microsatellite marker system validated in 56 A. dahuricaes.

loci No.	Motif	Primer Sequence(5'→3')		Tm (℃)	Allele Size (bp)	Na	Ne	Ho	He	I	PIC	HWE	Regions
1	(TA)8	F:	`GCCTCAAGAACTTGCCTCAC`	61	229–241	4	1.4323	0.6955	0.3018	0.5618	0.2702	0.1328	5’UTR
1	(TA)8	R:	`GTTTCACCACTTCTGCCACA`	61	229–241	4	1.4323	0.6955	0.3018	0.5618	0.2702	0.1328	5’UTR
2	(AG)9	F:	`CAGACCTGAATGGCACTGAA`	60	173–181	6	1.8518	0.5359	0.46	0.9361	0.4292	0.3709	5’UTR
2	(AG)9	R:	`TGAAGGAGTCTGCACACCAA`	60	173–181	6	1.8518	0.5359	0.46	0.9361	0.4292	0.3709	5’UTR
3	(TG)7	F:	`GATTGAGCAAGGAGAGGTGC`	60	241–250	4	1.7398	0.5709	0.4252	0.7959	0.3835	0.3024	5’UTR
3	(TG)7	R:	`CCATCAAATAAGAGGCACAAAA`	60	241–250	4	1.7398	0.5709	0.4252	0.7959	0.3835	0.3024	5’UTR
4	(CA)6	F:	`CAGCCGGCTAAGACAGAATC`	60	254–258	2	1.6228	0.6128	0.3838	0.5719	0.3101	0.2457	5’UTR
4	(CA)6	R:	`TGGGATCACTTTCTTGGCATA`	60	254–258	2	1.6228	0.6128	0.3838	0.5719	0.3101	0.2457	5’UTR
5	(TA)7	F:	`TTTTCATGGATGCACAATAAGC`	57	200–210	6	3.1266	0.3137	0.6802	1.304	0.6272	0.1465	5’UTR
5	(TA)7	R:	`GAAATTGATGGCATTCTCCC`	57	200–210	6	3.1266	0.3137	0.6802	1.304	0.6272	0.1465	5’UTR
6	(TC)9	F:	`TTGCAGCGTCAATTTGCTAC`	59	237–243	5	2.48	0.3978	0.5968	1.1452	0.5498	0.2736	5’UTR
6	(TC)9	R:	`AGAAAAAGTCCGGGCAAAGT`	59	237–243	5	2.48	0.3978	0.5968	1.1452	0.5498	0.2736	5’UTR
7	(AT)8	F:	`TTACGCAATTGCTGATTCCA`	58	212–220	3	1.9222	0.5159	0.4798	0.7084	0.3729	0.3362	5’UTR
7	(AT)8	R:	`CCCCGCTGGCTTATCTTTAT`	58	212–220	3	1.9222	0.5159	0.4798	0.7084	0.3729	0.3362	5’UTR
8	(AT)9	F:	`CCATGGAGAAATTGGCTGTT`	58	149–158	8	1.8909	0.5246	0.4711	1.0793	0.4521	0.0102	5’UTR
8	(AT)9	R:	`TTCTTGATCCAGTGATGCCA`	58	149–158	8	1.8909	0.5246	0.4711	1.0793	0.4521	0.0102	5’UTR
9	(GT)9	F:	`TTTGGGGCTGCTTTGTATTC`	59	269–285	3	1.7302	0.5742	0.422	0.6872	0.3528	0.6730	5’UTR
9	(GT)9	R:	`TCCAGCATCCAGTATCACCA`	59	269–285	3	1.7302	0.5742	0.422	0.6872	0.3528	0.6730	5’UTR
10	(TG)9	F:	`GGTTTGTGAGATCCTTTGGG`	60	237–243	4	1.682	0.5909	0.4055	0.6852	0.3427	0.6662	5’UTR
10	(TG)9	R:	`GAACTGACACTTGGGAATCCA`	60	237–243	4	1.682	0.5909	0.4055	0.6852	0.3427	0.6662	5’UTR

Na: Number of Different Alleles, Ne: Number of Effective Alleles, I: Shannon‘s Information Index, Ho: Observed Heterozygosity, He: Expected Heterozygosity, PIC: Polymorphism Information Content. HWE: Hardy-Weinberg equilibrium P-value (≥0.05).