FIGURE 1.
Inflammatory response of monocyte-derived Langerhans cells (LCs) towards heat-killed Staphylococcus aureus (HKSA) and keratinocyte-conditioned medium (K-CM). Immature cells were incubated in conditioned medium from control or HKSA-treated keratinocytes (A, C, D) or medium with 108 HSKA/mL (A, B, D, E). (A) Surface expression of HLA-DR and CD86 was analysed by flow cytometry. (B, C) Geometrical mean of fluorescence intensity for HLA-DR and CD86. (D) Gene expression was normalized to GAPDH before ratio on control conditions was calculated (E) Levels of pro-inflammatory cytokines in cell culture supernatants were measured by Multiplex. Data represent mean values ± SEM (n = 3–9). Paired t test with statistical data represented as *P < 0.05, **P < 0.01, ***P < 0.001