Figure 5. Effect of PnTx4(5-5) on L-Glu induced hyperalgesia. (A) Effect of intraplantar administration of PnTx4(5-5) (5 µg) on hyperalgesia induced by L-Glu (1 µg/paw). PnTx4(5-5) was administered 5 minutes after L-Glu. (B) Exclusion of systemic antinociceptive effect of PnTx4(5-5) at a dose of 5 µg. Toxin or saline were injected into the right paw (RP) 5 minutes after administration of L-Glu (1 µg) in both hind paws (RP/LP). Each column represents the mean ± S.E.M. of Δ nociceptive threshold (g) for four animals. *Indicates statistical significant difference (p < 0.05) from the control group (L-Glu + saline - R-Paw).