Table 2.

Averaged percent of parent populations positive for selected checkpoint markers in non-stimulated, 24 h, and 48 h stimulated populations in CD4⁺ and CD8⁺ cells.

CD4+
	PD-1+			CTLA-4+			ICOS+			TIM-3+			LAG-3+
	No stim	24 h	48 h	No stim	24 h	48 h	No stim	24 h	48 h	No stim	24 h	48 h	No stim	24 h	48 h
A	37.37	40.10	41.03	0.67	1.69	2.07	13.21	22.00	30.60	0.58	0.51	2.48	0.54	0.91	1.65
B	10.57	12.25	13.90	0.24	0.65	0.63	3.55	15.50	22.07	0.16	0.60	0.94	0.34	2.14	2.42
C	16.73	19.60	19.60	0.37	1.08	1.12	9.43	17.53	22.40	0.69	1.13	1.46	0.41	1.49	1.19
D	11.27	12.43	14.00	0.28	0.56	0.53	10.44	22.47	27.80	0.20	0.48	0.78	0.21	0.67	0.69
E	4.49	5.96	6.51	0.24	0.61	0.57	4.25	14.10	17.73	0.22	0.71	1.10	0.22	3.18	2.22
F	12.17	12.43	11.62	0.29	0.61	0.77	6.38	14.64	18.90	0.20	0.32	0.44	0.35	1.32	1.83
CD8+
	PD-1+			CTLA-4+			ICOS+			TIM-3+			LAG-3+
	No stim	24 h	48 h	No stim	24 h	48 h	No stim	24 h	48 h	No stim	24 h	48 h	No stim	24 h	48 h
A	52.77	55.73	52.90	0.26	0.42	0.54	1.02	1.17	2.60	1.32	2.73	9.95	0.42	2.49	3.45
B	15.07	14.03	15.67	0.18	0.21	0.22	0.15	1.00	1.46	0.38	1.55	2.84	0.09	4.40	4.39
C	11.60	13.00	12.10	0.24	0.35	0.39	0.77	1.63	2.41	0.97	2.20	2.91	0.36	2.37	1.54
D	18.10	19.27	21.17	0.19	0.36	0.29	0.56	2.03	2.37	0.51	2.19	4.36	0.23	1.89	2.40
E	6.86	7.77	9.20	0.18	0.27	0.22	0.26	1.35	2.19	0.50	2.82	5.33	0.18	10.89	8.94
F	11.60	11.57	10.69	0.23	0.28	0.30	0.19	0.60	0.81	0.30	1.12	2.57	0.21	2.57	3.49