Figure 5.

MEFs with and without over expression of SV40 Ta. A. Viable cell count (VCC) per cm² for MEFs with and without over expression of SV40 Ta. B. Viability (%) for MEFs with and without over expression of SV40 Ta. C. Doubling rate for MEFs with and without over expression of SV40 Ta. Cell lines transformed with SV40 Ta were stable with an average doubling rate of 27 h compared to an average doubling rate of 45 h in cultures without SV40 Ta. D. Presence of SV40 Ta confirmed by immunostaining with anti-SV40 Ta.