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. 2016 May 23;3(2):e41. doi: 10.14440/jbm.2016.110

Table 2.

qPCR temperature profile to determine telomere length.

Segment Temperature (°C) Time #Cycles Description
1 95 20 min 1 UDG & DNA polymerase activation
2 95 5 min 40 ds degradation
54 30 s Annealing and extension