Table 2.
Segment | Temperature (°C) | Time | #Cycles | Description |
1 | 95 | 20 min | 1 | UDG & DNA polymerase activation |
2 | 95 | 5 min | 40 | ds degradation |
54 | 30 s | Annealing and extension |
Segment | Temperature (°C) | Time | #Cycles | Description |
1 | 95 | 20 min | 1 | UDG & DNA polymerase activation |
2 | 95 | 5 min | 40 | ds degradation |
54 | 30 s | Annealing and extension |