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Comparison of median cell doubling rate and percent viability for various immortalization methods.
| Immortalization method | Serial passage | ROCK inhibitor | SV40 Ta |
| Cell doubling rate (h) Median ± SD | 45 ± 55 | 49 ± 37 | 25 ± 9 |
| Viability (%) Median ± SD | 92 ± 11 | 91 ± 12 | 94 ± 5 |
| Number of cell lines | n = 8 | n = 4 | n = 9 |
Note: MEFs were cultured with 3% O2 for each method.
