Table 2.
Sequence of human primers.
| Gene | Accession number | Sequences | Annealing temperature | Amplicon size |
|---|---|---|---|---|
| CXCR4 | AY242129.1 | For*: 5'-AATGAGGCCACAACAAACATCACA-3’ Rev*: 5‘-ATCCAGACGCCAACATAGAC-3' |
58°C | 464 bp |
| CD34 | M81104.1 | For: 5‘-AATGAGGCCACAACAAACATCACA-3' Rev: 5‘-CTGTCCTTCTTAACCTCCGCACAGC-3' |
56°C | 380 bp |
| HLA-DR | K01171.1 | For: 5‘-ATCATGACAAAGCGCTCCAACTAT-3' Rev: 5‘-GATGCCCACCAGACCCACAG-3' |
60°C | 404 bp |
| β-actin | NM_001101.4 | For: 5‘-GTCCTCTCCCAAGTCCACACA-3' Rev: 5‘-CTGGTCTCAAGTCAGTGTACAGGTAA-3' |
60°C | 240 bp |
*For, forward primer, Rev, reverse primer.