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. 2016 Jul 25;3(3):e50. doi: 10.14440/jbm.2016.124

Figure 2.

Figure 2.

Evaluation of potential neurotrophic strategies in SH-SY5Y cells. A. Standardised MTT assay of Garcinol-treated SH-SY5Y cells at 4 DIV. B. Graph showing the total neurite length/cell and representative photomicrographs of GFP-expressing GDF5-treated or caBMPRIb-transfected SH-SY5Y cells, compared to an untreated scramble-transfected control at 4 DIV. C. Graph showing relative immunofluorescence intensity and representative photomicrographs of ACH3 in SH-SY5Y cells treated for 24 h with either control or Garcinol. As indicated (**P < 0.01, ***P < 0.001 vs. control; One-way ANOVA with post-hoc Tukey’s test). Number of repetitions (N) = 3. Scale bar = 100 μm. D. Western blotting showing ACH3 protein levels in SH-SY5Y cells treated for 24 h with control or Garcinol. β-actin was used as a loading control. Graph showing quantification of ACH3 protein levels in relation to β-actin levels, as indicated (*P < 0.05 vs. control; Unpaired student t-test; N = 3).