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Example mass spectra (baseline-subtracted, smoothed, y-axis-autoscaled, and covering the mass range 2 kDa to 20 kDa) from solvent-optimization titrations, with 80% (v/v) acetonitrile, 2.5% (v/v) TFA, and saturated HCCA (A); 70% (v/v) acetonitrile, 2.5% (v/v) TFA, and saturated HCCA (B); 60% (v/v) acetonitrile, 2.5% (v/v) TFA, and saturated HCCA (C); and full-extraction control (D).
