Figure 1. Determination of optimal rapid chronological lifespan assay conditions.

Stationary phase aging yeast cultures were combined with MTT-containing assay solution and incubated. At the indicated times aliquots were removed, combined with solubilization reagent, and absorbance determined at 550 nm. A. Accumulation of formazan product in the presence or of menadione (+/-M) and glucose (+/-GLU). B. Accumulation of formazan product in presence of both menadione and glucose in the assay medium as a function of cell concentration (M = 10⁶). Error bars represent the standard error of three independent replicates.