Figure 3. Analysis of yeast chronological lifespan under conditions of caloric restriction.

Stationary phase aging yeast cultures in CM media containing either 2% or 0.2% glucose (GLU), or of cells suspended in water, were incubated for the indicated number of days (x-axis). Each day, an aliquot was removed from the culture and subjected to either the traditional CC assay (grey) or the novel RCL assay described here (black). For CC assays, viability is expressed as a percent of the mean cell number at day 0, for RCL assays as a percent of the mean absorbance at day 0 (y-axis). Error bars represent the standard error of three independent replicates.