Figure 8.

Hematoxylin and eosin (H&E) stain, (b) post hoc conventional B-mode image, and (c) T₂-weighted image of liquefaction in the same liver sample. The liquefaction zone (dotted black dotted outline in (a), red arrows) can be distinguished as hypoechoic in (b) and hyperintense in (c). Regions of more thorough liquefaction exhibit minimal H&E staining, (yellow arrow in (a)). Small hyperechoic structures can be seen in the post hoc conventional B-mode image (yellow arrows in (b)), possibly indicating residual bubbles or accumulations of cellular debris. The histotripsy pulse (1-MHz fundamental frequency, 5-μs pulse duration, 25-MPa peak negative pressure) propagated from left to right in each image.