Skip to main content
. Author manuscript; available in PMC: 2020 Aug 21.
Published in final edited form as: Neuron. 2019 Jun 26;103(4):627–641.e7. doi: 10.1016/j.neuron.2019.05.035

Figure 6. Vagotomy and α-syn deficiency prevent olfactory dysfunctions induced by α-syn PFF injection into the gut.

Figure 6.

(A-C) Olfactory behavioral assessments at 9 months after PBS and α-syn PFF gastrointestinal injection in WT (n=9–10), TV (n=7–8) and Snca−/− mice (n=10). The buried pellet trial test was performed for 4 days and the surface pellet control test was performed for one trial 1 day after the buried pellets. (A) Two pieces of sweetened cereal were buried along the perimeter of the cage approximately 0.5 cm below the bedding so that it was not visible. (B) Latency in finding the first pellet was recorded when the mouse touched the pellet. (C) The visible pellet trial test was set up in a similar way except that the piece of cereal was placed on top of the bedding. Error bars represent the mean ± S.E.M. Statistical significance was determined using a two-way ANOVA followed by post-hoc Bonferroni test for multiple group comparison. #P < 0.05, ##P < 0.01, ###P < 0.001 vs. PBS gastrointestinal injected WT group. *P< 0.05, **P < 0.01, ***P < 0.001 vs. α-syn PFF gastrointestinal injected WT group. n.s: not significant.