KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER | ||
|---|---|---|---|---|
| Antibodies | ||||
| Mouse anti-α-Synuclein | BD Bioscience | Cat#1610787; RRID: AB_398108 | ||
| Mouse anti-pSer129-α-synuclein | Biolegend | Cat#825701; RRID: AB_2564891 | ||
| Rabbit anti-pSer129-α-synuclein | Abcam | Cat#ab168381; RRID: AB_2728613 | ||
| Rabbit anti-Tyrosine Hydroxylase (TH) | Novus Biologicals | Cat#NB300–109; RRID: AB_10077691 | ||
| Rabbit anti-Dopamine transporter (DAT) | Sigma | Cat#D6944; RRID: AB_1840807 | ||
| Rabbit anti-Tuj1 | Biolegend | Cat#802001; RRID: AB_2564645 | ||
| Rabbit anti-Choline Acetyltransferase (ChAT) | Abcam | Cat#Ab178850; RRID: AB_2721842 | ||
| Mouse Anti-NeuN, clone A60, Cy3 Conjugate | Millipore | Cat#MAB377C3; RRID: AB_10918200 | ||
| Mouse anti-β-actin-HRP | Sigma | Cat#A3854; RRID: AB_262011 | ||
| Donkey Anti-Rabbit IgG, Whole Ab ECL Antibody, HRP Conjugated | GE Healthcare | Cat#NA934; RRID: AB_772206 | ||
| Sheep Anti-Mouse IgG, Whole Ab ECL Antibody, HRP Conjugated | GE Healthcare | Cat#NA931; RRID: AB_772210 | ||
| Donkey polyclonal anti-mouse Alexa fluor 488 | Jackson ImmunoResearch | Cat#715–545-151; RRID: AB_2341099 | ||
| Donkey polyclonal anti-rabbit Alexa fluor 488 | Jackson ImmunoResearch | Cat#711–545-152; RRID: AB_2313584 | ||
| Donkey polyclonal anti-mouse CY3 | Jackson ImmunoResearch | Cat#715–165-151; RRID: AB_2315777 | ||
| Donkey polyclonal anti-rabbit CY3 | Jackson ImmunoResearch | Cat#711–165-152; RRID: AB_2307443 | ||
| Chemicals, Peptides, and Recombinant Proteins | ||||
| 1X PBS pH 7.4 | Quality Biologicals | Cat#114–058-101 | ||
| 10X TBS pH 7.4 | Quality Biologicals | Cat#351–086-101 | ||
| Protease Inhibitor Cocktail | Sigma | Cat#P8340 | ||
| Phosphatase inhibitor cocktail 2 | Sigma | Cat#P5726 | ||
| Phosphatase inhibitor cocktail 3 | Sigma | Cat#P0044 | ||
| Paraformaldehyde (PFA) reagent grade | Sigma | Cat#P6148 | ||
| D.P.X. | Sigma | Cat#317616 | ||
| Thionin acetate salt | Sigma | Cat#T7029 | ||
| Normal donkey serum | Jackson ImmunoResearch | Cat#017–000-121 | ||
| VECTASHIELD HardSet Antifade Mounting Medium with DAPI | Vector laboratories | Cat#H-1500 | ||
| SIGMAFAST(TM) 3,3’-Diaminobenzidine tablets | Sigma | Cat#D4293 | ||
| [123I]ioflupane | Cardinal Health | DaTScan | ||
| Critical Commercial Assays | ||||
| Vectastain Elite ABC Kit (Peroxidase, Rabbit IgG) | Vector laboratories | Cat#PK-6101 | ||
| Vectastain Elite ABC Kit (Peroxidase, Mouse IgG) | Vector laboratories | Cat#PK-6102 | ||
| Pierce LAL chromogenic endotoxin quantitation kit | Thermo Scientific | Cat#88282 | ||
| Ghrelin (Rat, Mouse) EIA Kit, extraction-free | Phoenix Pharmaceuticals | Cat#EK-031–31 | ||
| PIERCE BCA PROTEIN ASSAY | Thermo Scientific | Cat#23227 | ||
| DreamTaq Green PCR Master Mix (2X) | Thermo Scientific | Cat#K1082 | ||
| Experimental Models: Organisms/Strains | ||||
| Mouse: B6;129X1-Sncatm1Rosl/J | Jackson Laboratory | JAX:003692 | ||
| Mouse: C57BL/6J | Jackson Laboratory | JAX:000664 | ||
| Oligonucleotides | ||||
| Primer: Mouse SNCA wild type forward: 5’-GGC GAC GTG AAG GAG CCA GGG A −3’ |
Jackson Laboratory | oIMR1286 | ||
| Primer: Mouse SNCA wild type reverse: 5’-CAG CGA AAG GAA AGC CGA GTG ATG TAC T-3’ |
Jackson Laboratory | oIMR1287 | ||
| Primer: Mouse SNCA mutant forward: 5’-CTG AAT GAA CTG CAG GAC GA-3’ |
Jackson Laboratory | oIMR158 | ||
| Primer: Mouse SNCA mutant reverse: 5’-ATA CTT TCT CGG CAG GAG CA-3’ |
Jackson Laboratory | oIMR159 | ||
| Recombinant DNA | ||||
| pRK172-mouse α-Synuclein | (Volpicelli-Daley et al., 2014) | N/A | ||
| pRK172-human α-Synuclein | (Volpicelli-Daley et al., 2014) | N/A | ||
| Deposited Data | ||||
| Raw data used for this study | This manuscript | DOI: 10.17632/swzrgbp3p5.1 | ||
| Software and Algorithms | ||||
| ImageJ | NIH | https://imagej.nih.gov/ij/ | ||
| Prism 6 | GraphPad software | https://www.graphpad.com/scientific-software/prism/ | ||
| ZEN lite | Zeiss | https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html | ||
| Stereo Investigator software | MBF Bioscience | http://www.mbfbioscience.com | ||
| ML-EM algorithm | MI Labs | reconstruction | ||
| AMIDE | SOURCEFORGE | http://amide.sourceforge.net/ | ||
| ANY-maze | Stoelting Co. | https://www.stoeltingco.com/anymaze.html | ||
| Other | ||||
| RIPA Lysis and Extraction Buffer | Thermo Scientific | Cat#89901 | ||
| 2X Lammli Sample Buffer | Bio-Rad | Cat#1610737 | ||
| Novex WedgeWell 8–16% Tris-Glycine Mini Gels | Thermo Scientific | Cat#XP08165BOX | ||
| SUPERSIGNAL WEST PICO PLUS | Thermo Scientific | Cat#34580 | ||
| Blotting-Grade Blocker, nonfat dry milk | Bio-Rad | Cat#1706404 | ||
| Restore Western blot stripping buffer | Thermo Scientific | Cat#21059 | ||
| Nitrocellulose Membrane | Bio-Rad | Cat#1620115 | ||
| X-ray film | RPI | Cat#248300 | ||
| Hamilton® GASTIGHT® syringe, 1700 series | Sigma | Cat#20972 | ||
| Pierce High Capacity Endotoxin Removal Spin Column | Thermo Scientific | Cat#88276 | ||
| Superdex 200 Increase 10/300 G | GE Healthcare | Cat#45–002-570 | ||
| Hitrap Q Sepharose Fast Flow | GE Healthcare | Cat#450–002-58 | ||
| Ni Sepharose 6 Fast Flow | GE Healthcare | Cat#17–5318-06 | ||