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Purification of HEL-oligomers by gel-filtration chromatography. After the reaction of Asp101-ss-PDE-HEL with SPDP-HEL, the mixture was applied to the column of Sephadex G-100. a. Elution profile of HEL-oligomers. b. A SDS-PAGE profile of HEL-oligomers in the indicated of the fractions in gel-filtration chromatography. Fractions from 35 to 42 were pooled, lyophilized, and used as HEL-oligomers.
