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Blood concentration of HEL-oligomers (PDE + SPDP). Sera were obtained from mice given a single administration with 100 μg HEL-oligomers (PDE + SPDP) on day −7 followed by immunization with HEL in CFA on day 0. On day 1 and 15, spleen cells were harvested from mice and the residual amount of HEL-oligomers in spleen cells were determined by competitive ELISA. Each point represents the mean concentration (M) and standard deviation of 3 mice.
