Table 2.

Biophysical properties in WT and IEM–related mutant, p.I136V, p.I848T, and p.1316 A, Na_v1.7 channels in three different temperatures.

Mutation (position)	Tested temperature (°C)	Activation		Fast inactivation				Area of window current
		Vh (mV)	k	Vh (mV)	k	τ, inactivation	τ, recovery from −80 mV (ms)
WT	25	−21.8 ± 1.5	8.3 ± 0.43	−85.7 ± 2.1	−12.5 ± 0.33		18.98 ± 0.34	1.13 ± 0.001
	40	−20.4 ± 1. 1	10.6 ± 0.32	−83.8 ± 1.9	−14.3 ± 0.34			1.51 ± 0.001
	15	−27.5 ± 1.2	6.1 ± 0.27	−76.9 ± 1.7	−13.7 ± 0.42		58.34 ± 0.23	1.78 ± 0.002
p.I136V	25	−27.5 ± 1.4	7.7 ± 0.23	−80.5 ± 1.2	−12.8 ± 0.21	↑	14.34	1.53 ± 0.001
(VSD)	40	−15.2 ± 1.2	7.5 ± 0.42	−67.0 ± 1.1	−13.8 ± 0.21	n.c.		1.78 ± 0.0001
	15	−36.8 ± 1.2	7.02 ± 0.34	−64.5±1.2	−13.2 ± 0.43	↑	108.52	3.86 ± 0.0001
p.I848T	25	−32.6 ± 1.2	7.6 ± 0.23	−73.5 ± 1.3	−12.5 ± 0.37	↑	18.39	2.38 ± 0.001
(S4–S5)	40	−18.5 ± 1.2	8.8 ± 0.23	−60.6 ± 1.4	−14.3 ± 0.12	n.c.		3.52 ± 0.001
	15	−41.5 ± 1.4	6.8 ± 0.21	−63.6 ± 1.2	−10.6 ± 0.24	↑	86.85	3.12 ± 0.0001
p.V1316A	25	−34.4 ± 1.3	8.7 ± 0.34	−77.1 ± 0.9	−11.9 ± 0.12	↑	20.93	2.0 ± 0.001
(S4–S5)	40	−41.7 ± 1.4	9.1 ± 0.32	−86.7 ± 0.8	−13.1 ± 0.32	n.c.		2.76 ± 0.0001
	15	−34.2 ± 1.5	7.4 ± 0.27	−68.7 ± 0.8	−12.3 ± 0.33	↑	129.61	4.57 ± 0.0001
Ref.		Figs 1 and 2, Fig. S1		Figs 1 and 2, Fig. S1		Fig. S2	Fig. 4; Fig. S6	Fig. 2, Fig. S1
Mutation (position)	Tested temperature (°C)	Resurgent current
		Peak (to I transient )	Ratio of I resurgent /I transient to WT at the same temperature	Time to peak	τ, decay	V h , activation (mV)	k, activation	τ, decay after depolarization at 60 mV (ms)	f 0 , decay after depolarization at 60 mV
WT	25	0.071 ± 0.003				44.5 ± 2.5	28.6 ± 1.5	64.2 ± 1.9	0.4 ± 0.03
	40	0.05 ± 0.01				44.7 ± 1.9	19.1 ± 1.5	n.d.	n.d.
	15	0.42 ± 0.03				44.8 ± 2.2	29.3 ± 1.3	21.4 ± 2.0	0.4 ± 0.02
I136V	25	0.12 ± 0.01	1.69 (0.12/0.071)	↑	↑	45.8 ± 2.2	29.0 ± 1.8	70.4 ± 1.6	0.45 ± 0.03
(VSD)	40	0.13 ± 0.01	2.6 (0.13/0.05)	↑↑	↑	44.7 ± 2.9	19.1 ± 1.9	n.d.	n.d.
	15	0.48 ± 0.02	1.14 (0.48/0.42)	↑	↑	37.6 ± 2.1	17.8 ± 1.2	29.4 ± 2.2	0.36 ± 0.01
I848T	25	0.19 ± 0.01	2.67 (0.19/0.071)	↑	↑	58.8 ± 2.17	30.3 ± 1.13	69.8 ± 1.5	0.55 ± 0.03
(S4–S5)	40	0.22 ± 0.01	4.4 (0.22/0.05)	↑↑	↑	49.1 ± 2.1	21.8 ± 1.2	n.d.	n.d.
	15	0.50 ± 0.05	1.19 (0.5/0.42)	↑	↑	34.6 ± 1.9	31.1 ± 1.4	53.6 ± 2.7	0.5 ± 0.03
V1316A	25	0.12 ± 0.01	1.5 (0.12/0.071)	↑	↑	45.8 ± 2.5	30.3 ± 1.9	86.0 ± 1.7	0.40 ± 0.05
(S4–S5)	40	0.17 ± 0.02	3.4 (0.17/0.05)	↑↑	↑	40.8 ± 2.3	21.7 ± 2.0	n.d.	n.d.
	15	0.34 ± 0.02	0.81 (0.34/0.42)	↑	↑	38.8 ± 2.0	36.7 ± 2.3	41.2 ± 2.0	0.40 ± 0.05
Ref.		Fig. 3	Fig. 3; Fig. S3	Fig. 3	Fig. 3	Fig. S4	Fig. S4	Fig. S5	Fig. S5

Activation or inactivation curves are shown using the Boltzmann function: 1/[1 + exp((V_h − V)/k)]. Arrows in the table are denoted the comparison between mutant and WT channel at the same temperature. (VSD = voltage sensing domain; WT = wildtype).

n.c.: not changed.; n.d.: not performed, the distribution was presented by mean ± S.E.M.