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. 2019 Aug 22;9:12252. doi: 10.1038/s41598-019-48697-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of biofilm matrix development by F. tularensis LVS and its surface structure mutants by CLSM. Panel (A) shows orthogonal sections of horizontal (z) and side views (x and y) of reconstructed three-dimensional biofilm images at a magnification of 25x. Nucleic acids were stained with Syto9 (green) and the matrix EPS stained with Texas Red-ConA (red). Panel (B) is an overview (x and y) view of the reconstructed three-dimensional z-stack of each biofilm growth on the coverslip. Panel C is the side view of the three-dimensional z-stack. Each picture was obtained from biofilms grown for 15 days.