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. 2019 Aug 22;4:18. doi: 10.1038/s41536-019-0079-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Vegfc/d-dependent lymphangiogenesis is dispensable for cardiac regeneration in adult zebrafish. TUNEL staining and AFOG staining were performed on vegfc^+/+:vegfd^+/+ (a) and vegfc^hy−/−:vegfd^−/− hearts (b) at 1 day after cryoinjury. Scale bar length: 200 μm (TUNEL) or 100 μm (AFOG). c Quantification of cell death in vegfc^+/+:vegfd^+/+ and vegfc^hy−/−:vegfd^−/− mutant hearts 1 day after cryoinjury. d Correlation analysis between myocardial volume and cell death in vegfc^+/+:vegfd^+/+ control and vegfc^hy−/−:vegfd^−/− mutant hearts at 1 day post cryoinjury. Black plots represent vegfc^+/+:vegfd^+/+ samples, green plots represent vegfc^hy−/−:vegfd^−/− samples. e Quantification of injury size in vegfc^+/+:vegfd^+/+ and vegfc^hy−/−:vegfd^−/− mutant hearts at 1 day after cryoinjury. f Correlation analysis between myocardial volume and injury size in vegfc^+/+:vegfd^+/+ control and vegfc^hy−/−:vegfd^−/− mutant hearts at 1 day post cryoinjury. Black plots represent vegfc^+/+:vegfd^+/+ samples, green plots represent vegfc^hy−/−:vegfd^−/− samples. Statistical analysis: unpaired, nonparametric, Mann–Whitney test was used to compare two means. Data are presented as the mean ± standard error of the mean (sem), n = 8 vegfc^hy−/−:vegfd^−/−, n = 7 vegfc^+/+:vegfd^+/+. g–i First column: picrosirius red and fast green staining was performed on vegfc^+/+:vegfd^+/+ and vegfc^hy−/−:vegfd^−/− hearts 6 months (180 days) after cryoinjury. Second column: tropomyosin staining was performed on vegfc^+/+:vegfd^+/+ and vegfc^hy−/−:vegfd^−/− hearts 6 months after cryoinjury. Third column: fibronectin staining was performed on vegfc^+/+:vegfd^+/+ and vegfc^hy−/−:vegfd^−/− hearts 6 months after cryoinjury. 30% of vegfc^hy−/−:vegfd^−/− hearts display an impaired regenerative response with collagen deposition and fibrosis evident at the injury site (*). Scale bar length: 0.8 mm. j Percentage of hearts showing complete or incomplete cardiac regeneration at 180 days post cryoinjury. Quantification of collagen content (k) or myocardial volume (l) in vegfc^+/+:vegfd^+/+ and vegfc^hy−/−:vegfd^−/− mutant hearts 6 months after cryoinjury. Black plots represent vegfc^+/+:vegfd^+/+ hearts, green plots represent vegfc^hy−/−:vegfd^−/− hearts that showed complete heart regeneration, red plots represent vegfc^hy−/−:vegfd^−/− hearts that had impaired cardiac regenerative capacity. Statistical analysis: unpaired, nonparametric, Mann–Whitney tests was used to compare two means. Data are presented as the mean ± standard error of the mean (sem), n = 8 vegfc^hy−/−:vegfd^−/−, n = 8 vegfc^+/+:vegfd^+/+. Significant differences are marked by an asterisk (*p < 0.05)