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. 2019 Aug 22;9:12284. doi: 10.1038/s41598-019-48678-0

Figure 1.

Figure 1

High-fat diet promotes tumour initiation and progression. (a) Survival curves of tumour-free ND-fed PyMT mice (n = 25) and HFD-fed PyMT mice (n = 28). (b) Body weights, tumour weights, total tumour volumes, and tumour counts of ND-fed PyMT mice (n = 13) and HFD-fed PyMT mice (n = 16) at 20 weeks of age. (c) Quantitative RT-PCR analysis of the mRNA levels of macrophage markers, proinflammatory cytokines, angiogenic markers, myeloid chemoattractants, Opn, Leptin, and Lepr in tumours harvested from ND-fed PyMT mice and HFD-fed PyMT mice at 20 weeks of age (n = 4 per group). (d) Quantitative RT-PCR analysis of Opn mRNA expression in fractions of tumours from ND-fed PyMT mice and HFD-fed PyMT mice after depleting adipocytes by centrifugation (n = 3 per group). Error bars indicate the s.e.m. *P < 0.05 and **P < 0.01.