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. 2019 Aug 22;9:12284. doi: 10.1038/s41598-019-48678-0

Figure 2.

Figure 2

CD11c+ M1-like macrophages are the major macrophage population in tumours, while CD206+ M2-like macrophages are the major resident macrophage population in the mammary gland. (a) Flow cytometric analysis of macrophage markers in mammary glands from WT mice at 20 weeks of age (n = 4). (b) Representative CD206 (red) immunostaining with DAPI (blue) staining of mammary glands from WT mice. (c) Quantitative RT-PCR analysis of the mRNA levels of macrophage markers and angiogenic markers in F4/80+ macrophages from the mammary glands of WT mice and from the tumour tissue of ND-fed PyMT mice (n = 3 per group). (d) Flow cytometric analysis of macrophage marker expression in tumours from ND-fed PyMT mice (n = 12) and HFD-fed PyMT mice (n = 11) at 20 weeks of age. (e) Quantitative RT-PCR analysis of Opn mRNA expression in F4/80+ macrophages from the tumours of ND-fed PyMT mice and HFD-fed PyMT mice (n = 3 per group). (f) Representative CD206 (green) and OPN (red) immunostaining with DAPI (blue) staining of tumours from ND-fed PyMT mice and HFD-fed PyMT mice. (g) Representative F4/80 (green) and OPN (red) immunostaining with DAPI (blue) staining of tumours from HFD-fed PyMT mice. Error bars indicate the s.e.m. *P < 0.05, **P < 0.01 and ***P < 0.001; NS, not significant.