Fig. 6.

Knockdown of pa-Eleanor(S) suppressed the long-range chromatin interaction without reducing FOXO3 gene activity and induced apoptosis in long-term estrogen deprivation (LTED) cells. a 3C-qPCR (chromosome conformation capture–quantitative polymerase chain reaction) analysis shows that pa-Eleanor(S) knockdown reduced interaction between ESR1 and b sites at 6q21 in LTED cells. Interaction frequencies were measured between the ESR1 promoter and indicated positions (a–d; thick blue bars in Fig. 2a). b Expression levels of genes around the Eleanor topologically associating domain with pa-Eleanor(S) knockdown in LTED cells. Quantitative reverse transcription polymerase chain reaction values of cells treated with Antisense LNA GapmeR for the negative control were set to 1. c Fluorescence-activated cell sorting (FACS) analysis of Annexin V-stained cells shows that knockdown of pa-Eleanor(S) increased apoptosis in LTED cells. A representative FACS profile is on the left. Quantification of the FACS analysis is on the right. Data presented in a–c are representative of three independent experiments (mean ± s.e.m.). P values were calculated using unpaired, two-tailed, Student’s t test (*P < 0.05, **P < 0.01, ***P < 0.001, n.d. = not detected in at least one experiment)