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. 2019 Aug 22;9:12234. doi: 10.1038/s41598-019-48561-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Photograph showing the arrangement of spinach leaf on the Bio-LSI chip (a), real-time imaging of O₂ reduction current without (b) and with illumination at 30klx (c). The illumination was turned off 10 min after the start of illumination and DCMU (5 μM) was added 10 min after turning off the light. Then, the illumination was turned on again after the addition of DCMU and subsequent images were taken at 0 min (d), 2 min (e), 4 min (f), 6 min (g), 8 min (h), 10 min (i) after turning on the light (at 28 min). (B) The change in reduction current measured on a single randomly selected electrode (indicated by a red open square in Fig. 8C).