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. 2019 Aug 22;9:12269. doi: 10.1038/s41598-019-48555-w

Figure 2.

Figure 2

Flow cytometry analysis in (Left) in BN16 cells treated with IF-B12-Cy5 (orange), B12-Cy5 (blue) (200 nM each) at 37 °C and IF-B12-Cy5 (200 nM) at 4 °C (green) in HBSS for 1 h. Untreated cell background fluorescence is indicated in red; (Right) J774A.1 cells treated with IF-B12-Cy5 (orange), B12-Cy5 (blue) (200 nM each) and IF-B12-Cy5 cells with mannan block (green) (2 mg/mL) in HBSS for 1 h at 37 °C.