Table 3.
Relaxant responses of bladder strips.
| Control | Dapagliflozin | HFD/STZ | HFD/STZ/dapa | |
|---|---|---|---|---|
| Isoprenaline | ||||
| pEC50 | 6.9 ± 0.3 | 7.1 ± 0.4 | 6.9 ± 0.4 | 7.2 ± 0.2 |
| Emax | 56 ± 9 | 56 ± 6 | 59 ± 11 | 60 ± 8 |
| Fenoterol | ||||
| pEC50 | 5.2 ± 0.3 | 6.2 ± 1.6 | 5.9 ± 1.6 | 5.9 ± 0.5 |
| Emax | 41 ± 15 | 65 ± 26 | 54 ± 28 | 58 ± 14 |
| 30 µM | 49 ± 13 | 62 ± 16 | 54 ± 19 | 53 ± 13 |
| CL 316,243 | ||||
| pEC50 | 7.8 ± 0.2 | 7.6 ± 0.1 | 7.9 ± 0.3 | 7.6 ± 0.2 |
| Emax | 80 ± 15 | 74 ± 8 | 78 ± 9 | 79 ± 5 |
| Forskolin | ||||
| 10 µM | -36 ± 18 | -25 ± 14 | -34 ± 11 | -22 ± 19 |
Data are means ± SD of 10 rats, except for HFD/STZ group (n = 9) with 1 strip being measured per rat for the β-adrenoceptor agonists and the mean of 1-3 strips for forskolin. Maximum relaxation responses (Emax) and those to 10 µM forskolin are expressed as % of tone, i.e. force prior to addition of first agonist concentration (“carbachol plateau”, see Table 2 ). A graphical depiction of the data is shown in Figure 5 .