FIG 5.

TbL11 interacts in vivo and in vitro with P34. (A) A whole-cell extract (WCE) was prepared from 3×Ty-TbL11 cells, and coimmunoprecipitations were performed using anti-P34/P37 antibody. The resulting immunoprecipitates were then analyzed via anti-Ty Western blotting. (B) Recombinant TbL11 was incubated with recombinant P34 in an anti-P34/P37 coimmunoprecipitation assay with or without addition of in vitro-transcribed 5S rRNA. Western blotting was performed using anti-His antibody. Blots are representative of three biological replicates, and average values with SDs were calculated. S, supernatant; P, pellet. A total of 10 μg of WCE was loaded in the WCE lane.