Supplementary figure 2.

SMAR1 and calnexin show inverse correlation. (a) MCF7 cells were treated with 5mM Tunicamycin in a time-dependant manner. Western blot analysis was done to check the expression of calnexin and SMAR1. β-Actin was used as loading control. (b) HCT116 p53+/+ cells were treated with 100 ng/ml EGF and real-time PCR was done to check the relative gene expression of calnexin. (c) 100 ng/ml Epidermal Growth Factor (EGF) treatment was given to HCT116 p53 +/+ cells and stained with calnexin antibody. DAPI was used for staining nucleus. (d) HCT116 p53+/+ cells were transfected with FLAG-SMAR1 followed by treatment with 100 ng/ml EGF and real-time PCR was done to check the relative gene expression of SMAR1. GAPDH was used as internal control for quantitative PCRs.