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. 2019 Aug 22;10:3779. doi: 10.1038/s41467-019-11560-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Overexpression of AGS changes spindle orientation and anchoring. a AGS-GFP overexpression (AGS-OE; 1 μg/μl) changed overall embryo morphology at the 16-cell stage. Images were obtained by immunofluorescence with methanol fixation that diminishes the live GFP signal. The AGS-GFP signal was thus visualized by anti-GFP antibody. At M-phase, spindle orientation was randomized and many of the spindle poles were anchored to the nearby cortex (arrowheads in the magnified view). ~50% of embryos showed phenotype, n = 14. b, c AGS-OE caused ectopic recruitment of microtubules labeled by mCherry-EMTB to the cortex (arrows) in over 80% of embryos, n = 25. Images taken are from live embryos. Each experiment was performed at least three independent times. Scale bars = 20 μm