. 2019 Aug 14;5(8):e02176. doi: 10.1016/j.heliyon.2019.e02176

Table 1.

The results of microbiological tests for GF status.

Aerobic/Anaerobic	Culture media	Culture temperature	Sample	before gross morphological experiments	before behavioral experiments	after behavioral experiments
Anaerobic	Cooked meat broth	37 °C	Fece	0/20	0/39	0/39
			Bedding	0/20	0/39	0/39
			Swab	0/20	0/39	0/39
			RO water in the bottle	0/20	0/39	0/39
		Room Temperature	Fece	0/20	0/39	0/39
			Bedding	0/20	0/39	0/39
			Swab	0/20	0/39	0/39
			RO water in the bottle	0/20	0/39	0/39
	Thioglycollate medium	37 °C	Fece	0/20	0/39	0/39
			Bedding	0/20	0/39	0/39
			Swab	0/20	0/39	0/39
			RO water in the bottle	0/20	0/39	0/39
		Room Temperature	Fece	0/20	0/39	0/39
			Bedding	0/20	0/39	0/39
			Swab	0/20	0/39	0/39
			RO water in the bottle	0/20	0/39	0/39
Aerobic	Heart infusion broth	37 °C	Fece	0/20	0/39	0/39
			Bedding	0/20	0/39	0/39
			Swab	0/20	0/39	0/39
			RO water in the bottle	0/20	0/39	0/39
		Room Temperature	Fece	0/20	0/39	0/39
			Bedding	0/20	0/39	0/39
			Swab	0/20	0/39	0/39
			RO water in the bottle	0/20	0/39	0/39
	Potate dextrose broth	Room Temperature	Fece	0/20	0/39	0/39
			Bedding	0/20	0/39	0/39
			Swab	0/20	0/39	0/39
			RO water in the bottle	0/20	0/39	0/39