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. 2019 Jul 12;17:701–713. doi: 10.1016/j.omtn.2019.06.025

Figure 5.

Figure 5

Effects of TRPC6 Overexpression or Knockdown on the Viability and Necrosis of HK-2 Cells under Conditions of H/R Injury with or without Nec-1 Treatment

(A) Cell viability was assessed using the CCK-8 assay. (B) Cells were stained with Annexin V-FITC (x axis) and PI (y axis), and analyzed by flow cytometry. (C) Statistical analysis of the percentage of necrotic cells (Annexin V−/PI+). *p < 0.05, compared with the siNC group; #p < 0.05 versus the pcDNA3.1 group; &p < 0.05 versus the Nec-1 + siTRPC6 group; @p < 0.05 versus the Nec-1 + pcDNA3.1 group.