Figure 8.

Nec-1 Pretreatment Protected Rat Kidney Tissue against I/R-Induced Oxidative Stress and Inflammation via the HIF-1α/miR-26a/TRPC6 Pathway

Rats in the I/R group were divided into five sub-groups according to whether OAG (Sigma, USA), SKF96365 (Sigma, USA), or veliparib (Selleck) was administered before the I/R procedure at 6 h. Similarly, rats in the I/R + Nec-1 group were also divided into three sub-groups. (A) Western blotting was performed to detect HIF-1α, RIP1, RIP3, TRPC6, PARP1, and Sirtuin-2 expression in renal tissues. (B) An MDA detection kit and NADP⁺/NADPH assay kit were used to measure the MDA level and NADP⁺/NADPH ratio, respectively. Specific ELISA kits were used to detect the levels of inflammatory cytokines, IL-1β, IL-10, and TNF-α. *p < 0.05 versus the control group; ^#p < 0.05 versus the I/R group; ^&p < 0.05 versus the I/R + Nec-1 group; ^@p < 0.05 versus the I/R + OAG group.