Table 1. Effects of methanol extract of N. sativa on insulin secretion from isolated rat islets.

Groups	Insulin secretion (ng/mg islet protein)
	3 mM Glucose	11 mM Glucose
Control (Glucose alone)	2.23 ± 0.65	5.31 ± 0.74
N. sativa (25 μg/ml)	2.61 ± 0.33	6.37 ± 0.47*
N. sativa (50 μg/ml)	3.67 ± 0.33*	6.91 ± 0.33*
N. sativa (100 μg/ml)	5.87 ± 0.45†	8.49 ± 0.85*
N. sativa (200 μg/ml)	6.77 ± 0.35†	9.97 ± 0.45†
Glibenclamide (10 μg/ml)	7.55 ± 0.25‡	10.31 ± 0.65†

Isolated rat islets were incubated for 60 min with methanol extract of N. sativa (25–200 μg/ml) in the presence of 3 or 11 mM glucose; whereas Glibenclamide (10 μg/ml) used as a reference control, respectively. Values are Mean ± SEM with n=4.

*P<0.05.

^†P<0.01.

^‡P<0.001 compared with control (3 and 11 mM glucose alone).