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. 2019 Aug 23;15(8):e9008. doi: 10.15252/msb.20199008

Figure 5. In vitro enzyme assays.

Figure 5

  1. Relative activity of NADP+‐dependent glucose‐6‐phosphate dehydrogenase in the presence of potential regulators; error bars represent the s.e.m. All regulators were tested at two concentrations in four distinct replicates (n = 4, ATP: 5 mM, 18 mM; GTP: 2 mM, 10 mM; IMP and AMP: 1 mM, 5 mM), and the asterisk denotes significant inhibition (18 mM ATP: P‐value = 0.029; 10 mM GTP: P‐value = 0.025, one‐tailed t‐test).
  2. Relative activity of phosphate acetyltransferase in the presence of phenylpyruvate and tryptophan; error bars represent the s.e.m. All regulators were tested at three concentrations in three distinct replicates (n = 3, phenylpyruvate: 0.1, 1, 5 mM; l‐tryptophan: 0.1, 1, 4.4 mM).
  3. Relative activity of fructose‐bisphosphate aldolase class II in the presence of 5 mM of potential regulators, except hypoxanthine, which was tested at 3.22 mM. All regulators were tested in three distinct replicates (n = 3), and error bars represent the s.e.m. The asterisk denotes significant activation (5 mM 3PG: P‐value = 0.025; 5 mM ATP: P‐value = 0.024; 5 mM PEP: P‐value = 0.046, one‐tailed t‐test).
Data information: The raw data for all in vitro enzyme assays can be found in Dataset EV3.