Figure 2.

Mice with transplantation tumor were treated with normal saline (NS), cis-Dichlorodiamineplatinum (Ⅱ) (DDP, 3 mg/kg), and 50, 100, 200 mg/kg of Ginkgo biloba exocarp extracts (GBEE). On the 18th day, tumor was removed and then tumor tissue section was prepared. (A) Effects of GBEE on the expression of CD34 in B16-F10 transplanted tumor. The level of CD34 in B16 transplanted tumor was determined by immunohistochemistry. The nucleu was dyed as blue, and CD34 was expressed in cytoplasm and was dyed as brown

(B) Effects of GBEE on MVD of B16-F10 transplanted tumor. The four most dense areas were selected and new blood vessel formation was calculated on the CD34 stained tumor tissue sections (×200). Microvessel density (MVD) was obtained by calculating the average microvessels in four fields. Data are shown as mean ± SD (n = 3). **P < 0.01, ***P < 0.001, vs. Model Control.