Table 5.

The Box-Behnken design and results of experiments.

		Parameters			Response
Run	A: Initial cholecalciferol concentration (IU/ml)	B: Tryptone (g/l)	C: Sucrose % (w/v)	D: Shaking speed (rpm)	Cholecalciferol per dry cell weight (IU/g)
1	250000.00	2.00	5.00	100.00	2629.61
2	250000.00	0.50	5.00	100.00	1321.96
3	250000.00	1.25	2.00	100.00	1144.07
4	100000.00	1.25	2.00	150.00	1566.53
5	250000.00	0.50	2.00	150.00	1228.04
6	250000.00	1.25	2.00	200.00	1405.95
7	250000.00	0.50	5.00	200.00	856.59
8	100000.00	0.50	5.00	150.00	1182.94
9	100000.00	2.00	5.00	150.00	1825.58
10	100000.00	1.25	5.00	100.00	1325.95
11	250000.00	1.25	5.00	150.00	3675.29
12	250000.00	1.25	5.00	150.00	3633.74
13	250000.00	1.25	5.00	150.00	3806.85
14	100000.00	1.25	8.00	150.00	1917.69
15	250000.00	2.00	5.00	200.00	1967.35
16	250000.00	0.50	8.00	150.00	1649.03
17	100000.00	1.25	5.00	200.00	785.331
18	400000.00	2.00	5.00	150.00	3880.96
19	400000.00	1.25	2.00	150.00	2263.93
20	250000.00	1.25	5.00	150.00	3629.65
21	400000.00	1.25	5.00	100.00	2380.49
22	250000.00	2.00	8.00	150.00	3753.4
23	400000.00	0.50	5.00	150.00	1775.68
24	400000.00	1.25	8.00	150.00	3625.97
25	250000.00	1.25	8.00	200.00	1659.39
26	250000.00	1.25	8.00	100.00	2751.53
27	250000.00	2.00	2.00	150.00	2234.16
28	250000.00	1.25	5.00	150.00	3580.94
29	400000.00	1.25	5.00	200.00	1921.96