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Superimposed microarray bioactivity chromatograms (MBCs) resulting from microarray bioassays of fractionated leupeptin (left) and aprotinin (right) injected at different concentrations to nanoLC. The MBCs are correlated with nanoLC-UV traces (bottom) obtained from measurement of leupeptin and aprotinin at 25 μM and 30 μM, respectively. For these analyses a short chromatographic gradient of 35 min was used.
