Figure 5.

Analysis of venoms of B. asper (left) and T. trigonocephalus (right) using the picofractionation platform employing profiling of plasmin inhibition and protease activity. B. asper shows a positive peak between retention time 28 and 33 min, indicating the presence of multiple venom proteases and/or plasmin activators. From the parallel obtained MS data, multiple masses are found to match the positive peak area of the microarray bioassay chromatogram (MBC) as shown as extracted-ion chromatograms (XICs). For T. trigonocephalus, both positive and negative peaks were observed indicating the presence of proteases and/or plasmin activators as well as plasmin inhibitors. The XICs indicate multiple potential bioactive peptides or proteins per peak.