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. Author manuscript; available in PMC: 2019 Aug 23.
Published in final edited form as: Reprod Toxicol. 2017 May 17;70:82–96. doi: 10.1016/j.reprotox.2017.05.005

Figure 5. Dose-dependent Embryo Dysmorphology following TNP-470 Exposure.

Figure 5.

(A) Rat Whole Embryo Culture. (B) Zebrafish Embryotoxicity Test. Gestation day (GD) 10 rat embryos and 1–4 hours post fertilization (hpf) zebrafish embryos were cultured under static conditions in the absence or presence of increasing concentrations of TNP-470 for two or five days, respectively, followed by evaluation of viability and morphology. A total of 18 or 12 separate structural features were evaluated in WEC and ZET, respectively (see methods for more details). TNP-470 exposure resulted in developmental abnormalities in the absences of lethality. The mean % of abnormal embryos is shown along with the samples sizes in the respective figures. Nonlinear regression analysis was used to estimate the concentration that caused a 50% incidence of abnormal embryos (EC50) using nonlinear curve fitting software. * Statistically different from the untreated group via 1-way ANOVA with Dunnett’s Multiple Comparison Test at P < 0.05.