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. 2019 Aug 22;38:369. doi: 10.1186/s13046-019-1369-7

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The effects of S1PR1 downregulation on cell proliferation and apoptosis of ESCC cells. a. CCK-8 assay analysis of kyse150 and TE-13 cells transfected with si-NC or si-S1PR1 respectively. b. Flow cytometry analysis of cell cycle distribution proportion in kyse150 and TE-13 cells transfected with si-NC or si-S1PR1 respectively. Representative images and average percentages of cells are shown. c. Colony formation assay of kyse150 and TE-13 cells transfected with si-NC or si-S1PR1 respectively. d. Flow cytometry analysis of apoptotic cells in kyse150 and TE-13 cells transfected with si-NC or si-S1PR1 respectively. The sum of Annexin V positive population and PI positive population is exhibited. e. Immunofluorescent staining of c-caspase3 of kyse150 and TE-13 cells transfected with si-NC or si-S1PR1 respectively. f. Western blot analysis of S1PR1, PCNA, cyclinD1, Bcl-xL, c-caspase3 and GAPDH in kyse150 and TE-13 cells transfected with si-NC or si-S1PR1 respectively