Fig. 6.

P-STAT3 inhibition inhibited proliferation and enhanced apoptosis with S1PR1 knockdown or overexpression in vitro. a Images of the xenograft tumors formed in BALB/c-Foxn1nu/Nju nude mice subcutaneously injected with TE-13 cells with S1PR1 knockdown or overexpression treated with/without SH-4-54. b TE-13 cells with S1PR1 knockdown or overexpression treated with/without SH-4-54. Tumor volumes of xenograft tumors are evaluated every 2 days. c Average weight of tumors derived from each group. d Body weight data for TE-13 xenograft mouse are evaluated every 2 days. e Western blot of S1PR1, PCNA, p-STAT3, STAT3 and c-caspase3 in xenografts from each group. Statistical significance was determined by Student’s t test. p < 0.05. f. H&E and immunostaining of S1PR1, p-STAT3, Ki-67 and TUNEL in xenografts from each group (scale bar, 100 μm). Statistical significance was determined by Student’s t test. p < 0.05