Figure 6.

Overview of the effect of both gene (SP-A1/SP-A2) product and of single gene product (SP-A2) on the regulation AM miRNome on both males and females, and males respectively. The diagram depicts comparison of miRNAs, target genes, and pathways after OxS in AM from mice carrying both (co-ex) SP-A1/SP-A2 variants (male and female, left, current study) and from mice carrying a single SP-A2 (male) gene variant (right) (41). The SP-A2 work has been published (41) and is used here for comparison reasons only. The significantly regulated miRNAs (23 and 11) in co-ex male and female and in SP-A2 male, respectively, in response to OxS were largely decreased (~92% in co-ex male, ~83% in co-ex female, and ~75% in SP-A2 male) with the exception of few miRNAs that were increased (see Figure 4). All the mRNA targets were upregulated in co-ex male and in SP-A2 and are shown in blue arrow (on the right). The mRNA targets in co-ex females were downregulated and are shown in pink arrow (on the left). The pathways that differ in SP-A2 and co-ex in response to OxS are shown in gray background and those that are similar between co-ex males and SP-A2 male are shown in white background.