Figure 3.

ddPCR detection of rAAV2RSM was not affected by the secondary structure of the vector genome or by the selection of the amplification target site. (A) ddPCR detection range of rAAV2RSM using ITR and SV40 as target sites. Eight ddPCR data points, corresponding to number of droplets ranging from 14,000 and 16,000, were plotted. The CV% values were <2.9% and 3.1% for ITR and SV40, respectively. (B) Comparison of the titer of rAAV2RSM vector genome treated with SmaI or not, as determined by qPCR and ddPCR. Linearized plasmid DNA was used as a reference standard for qPCR. Values are shown as mean ± SD from two independent experiments. CV%, percent coefficient of variation; ddPCR, droplet digital PCR; SD, standard deviation.