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. 2019 Aug 19;30(4):127–136. doi: 10.1089/hgtb.2019.031

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© Birei Furuta-Hanawa et al. 2019; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are cited.

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Figure 5. — Correlation between the integrity of rAAV2RSM evaluated by ddPCR and transfection activity. (A) rAAV2RSM was incubated at 37°C for 0, 1, 3, or 7 days and activity was detected as TU toward Vero cell by FACS analysis after 48 h of infection. The activity of rAAV was demonstrated as the ratio to day 0. (B) Correlation between ddPCR or qPCR titer and TU activity. The vector genome titers of rAAV2RSM were simultaneously detected by qPCR (SV40 titer using ScaI-digested pTR-UF11 as standard) and ddPCR (ITR⁺/SV40⁺ group). Values are indicated as mean ± SD (activity n = 3, titer n = 4). (C) The percentage of incomplete vector genome of treated rAAV2RSM. TU, transduction activity.