Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 19;30(4):127–136. doi: 10.1089/hgtb.2019.031

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Birei Furuta-Hanawa et al. 2019; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are cited.

PMC Copyright notice

Figure 6. — Plasmid impurity of the rAAV vector products detected by 2D ddPCR. (A) Gene structure of positive strand of rAAV, dashed line is the plasmid backbone linked with the 5′ end and 3′ end of the ITR sequence. Narrow and thick arrows are indicated as the position of ITR and ori (replication origin) primer probe target site in this research, respectively. (B, C) Dot plot profiles of FAM-labeled ori primer probe (channel 1, amplitude) and HEX-labeled ITR primer probe (channel 2, amplitude). Droplets of 2D signals were separated into four groups. Ratio of each group is indicated in percentage. (B) rAAV2RSM. (C) rAAV8RSM. rAAV8RSM, rAAV reference standard material for serotype 8.