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. Author manuscript; available in PMC: 2019 Aug 23.
Published in final edited form as: Bioconjug Chem. 2019 Jul 5;30(7):2049–2059. doi: 10.1021/acs.bioconjchem.9b00332

Figure 7.

Figure 7.

Adoptive diabetes transfer experiment using bioconjugate treated diabetic splenocytes. Twenty million recent diabetic female NOD mice (n = 4) splenocytes were treated with F4/80 Ab liposome or GAD541–554BC with F4/80 Ab (50 μg/mL) or APL9BC with F4/80 Ab (50 μg/ mL) and transferred into young irradiated NOD mice (n = 6 for each variable). The positive control was left untreated. (A) Percentage of nondiabetic adoptive transfer recipient mice at the end of the experiment. Mice with a blood glucose level of ≥250 mg/dl were considered diabetic. Log-Rank analysis was used: n = 6, *P ≤ 0.05, **P ≤ 0.01. (B) Percentage of insulin positive islets determined based on the nuclei (blue) and insulin (green) labeling. One way ANOVA followed by Tukey’s multiple comparison test were used: n = 3, **P ≤ 0.01, ***P ≤ 0.001.