Table 1.

Primer sequences of target genes coding for antigenic proteins, used in this study.

	PCR end-point			Real-time PCR
Gene locus	Sequences (5′ – 3′)	Ta/°C	Size (bp)	Sequences (5′ – 3′)	Ta/°C	Size (bp)
A. peg-1	F: ATCCTCTTCACATTCGCTTT	57 °C	639	F: CATGTGCCGATAAATGCGGG	57 °C	130
	R: GGATAATAATGGTCGGGCAA			R: CCCTGTGAGCATGCATCCTT
A. peg-7	F: ACACCTCCATCTGAACAAA	57 °C	899	F: TATCGGAATGCGTGACTGCA	57 °C	130
	R: CCTAACATGCAGGCGATTA			R: AGGCAGTTTCCATGGTGTATG
A. peg-13	F: CATGAAATCACTCGAACACG	55 °C	931	F: AAACATTCGACGCCTACACC	60 °C	108
	R: TGTTCCTCCTTGTGCTCT			R: CATCGTGGTCTTCTCTGCGA
GAPDH	–	–	–	F: CCCCTTCATCAACATCGACT	60 °C	152
				R: TCAGCTCCCCATTTGATTTC

GAPDH, glyceraldehyde-3-phosphate dehydrogenase (housekeeping gene); PCR, polymerase chain reaction.