Fig. 3.

Monoterpene production by canonical terpene synthases. Five different monoterpene synthases were overexpressed in yeast cells producing solely GPP (brown), or also producing NPP as an alternative substrate (green). The monoterpene product titers increased markedly when NPP was also available with all synthases tested: Salvia fruticosa 1,8-cineole synthase (SfCinS1) (a), Pinus taeda α-pinene synthase (PtPinS) (b), Citrus limon limonene synthase (ClLimS) (c), S. pomifera sabinene synthase (SpSabS) (d), and S. elaeagnifolium camphene synthase (SeCamS) (e). A significant shift from camphene to limonene was observed in the product profile of SeCamS with NPP. Yeast cells producing NPP in the absence of any monoterpene synthase are shown as control (blue). Samples were analyzed in triplicate (n = 3 biologically independent samples). Error bars for each sample correspond to the mean absolute deviation (MAD) around the mean. Source data of a–e  are provided as a Source Data file